In response to our 2024 Spring Pilot Sequencing Program, we are pleased to announce our two awardees of the program:
- Owen Tamplin, Assistant Professor in the Department of Cell & Regenerative Biology
- Jesse Weber, Assistant Professor in the Department of Integrative Biology
Support for this program was made possible by partnerships with Illumina and 10X Genomics. Please join us in thanking our local reps- Logan Silber and Robin Ball of Illumina, and Eve-Marie Walter of 10X Genomics.
The UWBC 10X Genomics & Illumina Sequencing Pilot Program supports the use of single cell transcriptomic research with high-throughput sequencing solutions for genomics research at the University of Wisconsin. The goal of this program is to enable researchers to generate pilot data to submit in support of future grant proposals and to promote the use of the cutting-edge genomic solutions available at the UWBC.
To help UW investigators generate preliminary data, the UWBC has partnered with 10X Genomics to support single-cell pilot projects & Illumina to subsidize reagents for library prep and sequencing.
- 10X Genomics: 10X Genomics will provide two reactions of GEM-x 3’ or 5’ gene expression and Flex reagents at no cost. This can be combined with the no-cost sequencing reagents.
- Illumina: Illumina will provide reagents for a single 10B lane (1.25B reads) of 2x150bp sequencing at no cost.
Key Dates
| Application open date | Friday, May 17, 2024 |
| Application due | Friday, May 31, 2024, 3PM |
| Pilot awards announced | Friday, June 7, 2024 |
| Sample submission deadline | Friday, October 25, 2024 |
Download application here.
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Eligibility
- UW Principal Investigators (PIs) with faculty positions and applying for a future grant as the PI.
- Post-docs and doctoral researchers with funding managed separately from their supervising PI and applying for a future grant as the PI.
- Applicants can be from any UW System campus.
- Applicants should not hold current awards directly related to the proposed research.
Note:
- Multiple applications will be accepted, but only one can be approved per applicant.
- Applicants are responsible for single cell sample preparation. Evaluation of mock sample preps are available upon request.
Review Criteria
A review panel of UWBC experts will be responsible for evaluating eligible applications. 10X Genomics will assist in reviewing applications.
- Primary factor will be the technical feasibility of the proposed project.
- Consideration will be given to new UW PIs.
- No critiques will be provided to applicants.
- Applicants will be informed on Friday, June 7, if their application was approved and what components are subsidized (i.e. sample prep reagents, sequencing).
Approved Pilot Project Requirements
- Support the balance of the pilot costs. Labor & Setup charges are not included. Additional estimated cost ~$4,000.
- Provide follow-up information regarding the long-term impact of the pilot on your overall research and funding. This will assist the UWBC in generating outcome metrics for supporting future pilot programs.
- Agree to use the UWBC for library preparation and next-gen sequencing needs when the full proposal is funded.
- Acknowledge the “University of Wisconsin Biotechnology Center” in publications.
Application Resources
Please see the below resources for guidance on the various elements that go into a successful single cell RNA-seq experiment:
The Gene Expression Center’s “Guide to Getting Started with 10X Genomics”
The GEC’s Overview of the Gene Expression Flex / Fixed RNA Profiling Assay
10X Genomics Grant Application Resource: Single Cell Gene Expression (n.b. 10X has not yet updated this document to reflect the specific changes for the new “GEM-X” assay versions.)
10X Genomics – Cell Preparation for Single Cell Protocols – This document is a great general resource for preparing single cell suspensions that are compatible with 10X Genomics assay chemistry.
10X Genomics Sample Prep – This page contains links to a variety of example sample prep protocols in addition to guidance for other common aspects of scRNA experiments (e.g. flow sorting considerations; dead cell removal; etc.)