In collaboration with the DNA Sequencing Facility the GEC offers RNA library preparation services for sequencing on the Illumina NovaSeq 6000 platform.
The following RNA library preparation services are available:
QIAseq miRNA Library Kit
TruSeq Stranded Total & mRNA
-Stranded mRNA: polyA enrichment
-Stranded total RNA: Ribosomal RNA depletion. Available in Plant and H/M/R (“Gold”) subtypes
-Ribo-Zero Plus (H/M/R + B. subtilis/E. coli rRNA; globin reduction) Ribosomal RNA depletion. Compatibility with additional species possible with custom probes.
SMART-Seq® v4 Ultra® Low Input RNA / Nextera XT (ultra-low input) – polyA enrichment
SMARTer® Stranded Total RNA Sample Prep Kit – Low Input Mammalian (ultra-low input) – random hexamer reverse transcription; rRNA depletion of cDNA
SMARTer Stranded Total RNA-HI Mammalian (Human/Mouse/Rat rRNA reduction) Alternative to TruSeq Stranded Total RNA
We request all new users of our services please contact gecinfo@biotech.wisc.edu to set up a meeting to discuss the proposed project.
GETTING STARTED
To discuss a project or obtain a quote please e-mail gecinfo@biotech.wisc.edu.
Please review our updated sample drop-off guidelines (effective June 2021): GEC Sample Exchange Policy
SAMPLE SUBMISSION
Samples may be submitted on Tuesday and Thursday between 9-10AM by appointment.
Appointments may be made by e-mailing gecinfo@biotech.wisc.edu.
All sample submissions must include completed electronic copies of both the RNA-Seq Submission Form and Sample Submission sheet. Sample submission guidelines are available in the Sample Submission sheet. If you have any questions please let us know.
DOCUMENTS FOR DOWNLOAD
Illumina RNA-seq Submission Form
GEC Sample Submission Spreadsheet
DATA ANALYSIS
The UWBC Bioinformatics Resource Center offers data analysis support for all RNA Seq projects processed by the Gene Expression Center.
ACKNOWLEDGING UNIVERSITY OF WISCONSIN-MADISON BIOTECHNOLOGY CENTER
A visible measure of a core’s impact is made through proper acknowledgement of usage in publications. To acknowledge our core, please use the following statement:
“The author(s) utilized the University of Wisconsin – Madison Biotechnology Center’s Gene Expression Center Core Facility (Research Resource Identifier – RRID:SCR_017757) for RNA library preparation and the DNA Sequencing Facility (RRID:SCR_017759) for sequencing.”
To ensure you are receiving the most up-to-date pricing information, please contact us at gecinfo@biotech.wisc.edu to request a quote. Thank you!
| UW System Rates |
Non UW System Rates | ||||
| Library Type | Details | Samples 1-48 |
Samples 49-96 |
Samples 1-48 |
Samples 49-96 |
| TruSeq Stranded mRNA | Sample 1 | $1,865 | $2,140 | $2,352 | $2,840 |
| Each additional sample | $115 | $115 | $127 | $127 | |
| TruSeq Stranded Total RNA (Gold) |
Sample 1 | $1,930 | $2,205 | $2,430 | $2,920 |
| Each additional sample | $180 | $180 | $220 | $220 | |
| Samples 1-24 |
Samples 1-24 |
||||
| QiaSeq miRNA | Sample 1 | $1,650 | $2,075 | ||
| Each additional sample | $125 | $135 | |||
| Samples 1-48 |
Samples 49-96 |
Samples 1-48 |
Samples 49-96 |
||
| Takara SMARTer v4 / NexteraXT | Sample 1 | $1,690 | $1,865 | $2,300 | $2,750 |
| Each additional sample | $140 | $140 | $145 | $145 | |
| Takara SMARTer Stranded Total RNA Pico v2 Mammalian |
Sample 1 | $1,685 | $2,035 | $2,055 | $2,420 |
| Each additional sample | $110 | $110 | $130 | $130 | |
| RNA QC- Agilent & NanoDrop |
|||||
| RNA NanoChip (up to 12 samples) | $171 | $264 | |||
| RNA PicoChip (up to 11 samples) | $175 | $270 | |||
| Small RNA (up to 11 samples) | $190 | $295 | |||
| DNA1000 Chip (up to 12 samples) | $173 | $267 | |||
| HS DNA Chip (up to 11 samples) | $201 | $311 | |||
All data and services provided by the University of Wisconsin Biotechnology Center Gene Expression Center are intended for research purposes only. Services are not intended nor certified for diagnostic or clinical use.