RNA Extraction Service

  • Can start from any sample type (tissue, cells, blood, swabs, etc…)
  • Semi-automated extraction using Qiagen TissueLyser
  • Organic extraction with cleanup using Qiagen RNAeasy kit if necessary
  • Specific protocol optimized for each sample type
  • RNA may then be used for sequencing or arrays or returned to the user for their own assays

Please contact gecinfo@biotech.wisc.edu for a quote for your project. Cost varies depending on extraction method required to extract good quality RNA from your samples.

SUBMISSION:

Please contact gecinfo@biotech.wisc.edu prior to sample collection if possible

The best method of sample collection is immediate flash-freezing followed by storage at -80C. RNA preservatives such as RNA Later can be used but may interfere with processing of the samples.

We request 2-3 representative samples for optimization purposes.

When submitting samples you must include a digital copy of the RNA Extraction Submission Form and excel sample spreadsheet with sample names e-mailed to gecinfo@biotech.wisc.edu.

Please review our updated sample drop-off guidelines (effective June 2021): GEC Sample Exchange Policy

DOCUMENTS FOR DOWNLOAD:

RNA Extraction Submission Form
GEC Sample Submission Spreadsheet

RNA QC:

All RNA is checked for quality on the Agilent Bioanalyzer or Tapestation and purity on the NanoDrop One Spectrophotometer. Qubit measurements are available upon request and may be performed for cases where the NanoDrop is insufficient. Results of the QC will be emailed to the user upon completion of the extraction. QC results will be approved by the user prior to continuation of any assays

Sample type  UW System
Rate
 External Rate
 Tissue
 Sample 1  $580  $900
 Per sample  $15  $18
 Cell Pellet  Sample 1  $300  $465
 Per sample  $10  $12

Please contact gecinfo@biotech.wisc.edu for a quote. Cost varies depending on extraction method required to extract good quality RNA from your samples.

All data and services provided by the University of Wisconsin Biotechnology Center Gene Expression Center are intended for research purposes only. Services are not intended nor certified for diagnostic or clinical use.