QC Services
Standard RNA QC Service includes NanoDrop and Agilent analysis of samples to assess concentration/purity and integrity, respectively. Agilent DV200 Analysis is also available upon request.
Fluorometric quantification of RNA & cDNA is also available upon request.
QC Services will occur within 1-3 business days upon receipt of samples.
Please use the UWBC Request Portal to request our QC Service.
Sample submission guidelines for RNA QC Service
- Please submit a minimum of 4uL in nuclease-free 0.2mL strip-tube(s). Caps should be labeled 1-XXX. PI name & date must be on one or two tubes in the strip. If Qubit quantification is also requested, we request 5uL.
- Sample Submission sheet must contain sample IDs that match tube cap number.
Samples will be QC’d within 1-3 business days. Each RNA sample will be assayed on the NanoDrop One Spectrophotometer and Agilent Tapestation to assess concentration/purity and integrity, respectively.
Recommended A260/A280 ratio range 1.8 – 2.1; A260/A230 ratio should be similar indicating pure nucleic acids.
Note– 260nm and 280nm are the absorbance wavelengths used to assess the purity of RNA and DNA
The A260/A280 ratio is used to assess the presence of protein and or phenol in the RNA sample.
The A260/A230 is used to indicate the presence of unwanted organic compounds such as Trizol, phenol, Guanidine HCL and guanidine thiocyanate that are carried over from the RNA extraction procedure.
Samples are discarded upon completion of QC, unless client clearly communicates samples are to be held for future downstream service.
RNA QC Service
| Agilent 4200 Tapestation Service | UW System Rate | Non-UW System Rate |
| RNA QC (HT)- 96 | $775 | $892 |
| RNA QC (HT)- 72 | $650 | $748 |
| RNA QC (HT)- 48 | $445 | $552 |
| RNA QC (LT)- 24 | $320 | $368 |
| RNA QC (LT)- 12 | $115 | $133 |
| Agilent 2100 Bioanalyzer Service | ||
| RNA NanoChip (1-12 samples) | $115 | $272 |
| RNA PicoChip (1-11 samples) | $185 | $269 |
| HS DNA Chip (1-11 samples) | $225 | $259 |
| D1000 Chip (1-12 samples) | $182 | $210 |
| Qubit v4.0 Quantification Service | UW System Rate |
Non-UW System Rate |
| 1X HS dsDNA per sample |
$3 |
$3.50 |
| HS RNA per sample |
$3 |
$3.50 |
| RiboGreen Quantification Service (96-well format) | UW System Rate |
Non-UW System Rate |
| 24 samples |
$125 |
$195 |
| 48 samples |
$175 |
$273 |
| 72 samples |
$300 |
$467 |
| 96 samples |
$375 |
$583 |
Quant-iT RiboGreen RNA Quantitation Range 1-200ng (2uL sample used per assay)
Pricing current as of June 2023.
All data and services provided by the University of Wisconsin Biotechnology Center Gene Expression Center are intended for research purposes only. Services are not intended nor certified for diagnostic or clinical use.