10X Genomics recently launched new versions of their flagship 3′ and 5′ Gene Expression assays integrating an approach they call “On-Chip Multiplexing” or OCM. The OCM assays feature a relatively very low per sample reagent …
Month: February 2025
Sample Multiplexing vs. Library Multiplexing in 10X Genomics Single Cell RNA-seq
The below diagram illustrates the flow of a set of cell suspension samples through the process of library preparation and sequencing. A cell suspension, which can either be its own unique sample or (optionally) a …
Sample Prep for Single Cell/Nuclei RNA-seq
With rare exception, the single most important factor in determining the success of a single cell RNA-seq library preparation is the condition and health of the single cell/nuclei suspension at the time their transcripts are …