The below diagram is a representation of a barcoded double-stranded cDNA molecule produced from the 10X Genomics 3′ Gene Expression assay. This 5-minute video shows how Illumina’s “sequencing by synthesis” approach works and may be …
Month: March 2025
Interpreting the “fraction of reads in cells” values reported with the MiSeq results in single cell RNA-seq
In short: the “fraction of reads in cells” value is a measure of the proportion of sequencing reads assigned to (likely) true cells versus reads attributed to “background” such as RNA from dying/lysed cells captured …